stitch multiple microscope images using python












2














I'm trying to stitch a scan of a large area made using a microscope but I'm not having a whole lot of luck.

I started testing with 3 images extracted from a scanning video.
I stitched each 2 matching images but when I want to combine the 2 new composite images it looks very warped.
Does anyone know a decent way to stitch multiple images scanning a surface by changing the perspective but not the angle of view? I've only found ways that assume that the perspective remains constant and you are just rotating around that
enter image description hereenter image description hereenter image description here



Alternative to this problem: is it possible to align images using keypoints without warping?



edit: I've got it working now without any distortions but does anyone know a way to merge images while ignoring black? I want to get rid of these black lines beneath the image.
enter image description here



Edit: Problem solved.
enter image description here










share|improve this question
























  • Nice job. Have you by chance open sourced the code you have accomplished this with?
    – creimers
    Jan 7 at 17:09










  • It's a bit messy but I can give you a copy if you want
    – Arno van der Weijden
    Jan 7 at 19:52










  • That would be great. How would you prefer to share the copy?
    – creimers
    2 days ago










  • It's messy code but it works....we.tl/t-whtVSIWRFc My boss has the full version as an exe but I can't find the code for it anymore. I simply had opencv create a border around each strip that cut slightly into the image itself and removed everything outside of that. After that I had it overlay the strips and allowed for some manual adjustments (no deformations since that would cause major distortions due to the time difference between measuring each strip)
    – Arno van der Weijden
    11 hours ago












  • There are some minor distortions in each strip but they are very minor according to my measurements.
    – Arno van der Weijden
    11 hours ago
















2














I'm trying to stitch a scan of a large area made using a microscope but I'm not having a whole lot of luck.

I started testing with 3 images extracted from a scanning video.
I stitched each 2 matching images but when I want to combine the 2 new composite images it looks very warped.
Does anyone know a decent way to stitch multiple images scanning a surface by changing the perspective but not the angle of view? I've only found ways that assume that the perspective remains constant and you are just rotating around that
enter image description hereenter image description hereenter image description here



Alternative to this problem: is it possible to align images using keypoints without warping?



edit: I've got it working now without any distortions but does anyone know a way to merge images while ignoring black? I want to get rid of these black lines beneath the image.
enter image description here



Edit: Problem solved.
enter image description here










share|improve this question
























  • Nice job. Have you by chance open sourced the code you have accomplished this with?
    – creimers
    Jan 7 at 17:09










  • It's a bit messy but I can give you a copy if you want
    – Arno van der Weijden
    Jan 7 at 19:52










  • That would be great. How would you prefer to share the copy?
    – creimers
    2 days ago










  • It's messy code but it works....we.tl/t-whtVSIWRFc My boss has the full version as an exe but I can't find the code for it anymore. I simply had opencv create a border around each strip that cut slightly into the image itself and removed everything outside of that. After that I had it overlay the strips and allowed for some manual adjustments (no deformations since that would cause major distortions due to the time difference between measuring each strip)
    – Arno van der Weijden
    11 hours ago












  • There are some minor distortions in each strip but they are very minor according to my measurements.
    – Arno van der Weijden
    11 hours ago














2












2








2







I'm trying to stitch a scan of a large area made using a microscope but I'm not having a whole lot of luck.

I started testing with 3 images extracted from a scanning video.
I stitched each 2 matching images but when I want to combine the 2 new composite images it looks very warped.
Does anyone know a decent way to stitch multiple images scanning a surface by changing the perspective but not the angle of view? I've only found ways that assume that the perspective remains constant and you are just rotating around that
enter image description hereenter image description hereenter image description here



Alternative to this problem: is it possible to align images using keypoints without warping?



edit: I've got it working now without any distortions but does anyone know a way to merge images while ignoring black? I want to get rid of these black lines beneath the image.
enter image description here



Edit: Problem solved.
enter image description here










share|improve this question















I'm trying to stitch a scan of a large area made using a microscope but I'm not having a whole lot of luck.

I started testing with 3 images extracted from a scanning video.
I stitched each 2 matching images but when I want to combine the 2 new composite images it looks very warped.
Does anyone know a decent way to stitch multiple images scanning a surface by changing the perspective but not the angle of view? I've only found ways that assume that the perspective remains constant and you are just rotating around that
enter image description hereenter image description hereenter image description here



Alternative to this problem: is it possible to align images using keypoints without warping?



edit: I've got it working now without any distortions but does anyone know a way to merge images while ignoring black? I want to get rid of these black lines beneath the image.
enter image description here



Edit: Problem solved.
enter image description here







python images opencv photo-stitching






share|improve this question















share|improve this question













share|improve this question




share|improve this question








edited Jul 6 '18 at 14:34







Arno van der Weijden

















asked Jul 5 '18 at 10:26









Arno van der WeijdenArno van der Weijden

11817




11817












  • Nice job. Have you by chance open sourced the code you have accomplished this with?
    – creimers
    Jan 7 at 17:09










  • It's a bit messy but I can give you a copy if you want
    – Arno van der Weijden
    Jan 7 at 19:52










  • That would be great. How would you prefer to share the copy?
    – creimers
    2 days ago










  • It's messy code but it works....we.tl/t-whtVSIWRFc My boss has the full version as an exe but I can't find the code for it anymore. I simply had opencv create a border around each strip that cut slightly into the image itself and removed everything outside of that. After that I had it overlay the strips and allowed for some manual adjustments (no deformations since that would cause major distortions due to the time difference between measuring each strip)
    – Arno van der Weijden
    11 hours ago












  • There are some minor distortions in each strip but they are very minor according to my measurements.
    – Arno van der Weijden
    11 hours ago


















  • Nice job. Have you by chance open sourced the code you have accomplished this with?
    – creimers
    Jan 7 at 17:09










  • It's a bit messy but I can give you a copy if you want
    – Arno van der Weijden
    Jan 7 at 19:52










  • That would be great. How would you prefer to share the copy?
    – creimers
    2 days ago










  • It's messy code but it works....we.tl/t-whtVSIWRFc My boss has the full version as an exe but I can't find the code for it anymore. I simply had opencv create a border around each strip that cut slightly into the image itself and removed everything outside of that. After that I had it overlay the strips and allowed for some manual adjustments (no deformations since that would cause major distortions due to the time difference between measuring each strip)
    – Arno van der Weijden
    11 hours ago












  • There are some minor distortions in each strip but they are very minor according to my measurements.
    – Arno van der Weijden
    11 hours ago
















Nice job. Have you by chance open sourced the code you have accomplished this with?
– creimers
Jan 7 at 17:09




Nice job. Have you by chance open sourced the code you have accomplished this with?
– creimers
Jan 7 at 17:09












It's a bit messy but I can give you a copy if you want
– Arno van der Weijden
Jan 7 at 19:52




It's a bit messy but I can give you a copy if you want
– Arno van der Weijden
Jan 7 at 19:52












That would be great. How would you prefer to share the copy?
– creimers
2 days ago




That would be great. How would you prefer to share the copy?
– creimers
2 days ago












It's messy code but it works....we.tl/t-whtVSIWRFc My boss has the full version as an exe but I can't find the code for it anymore. I simply had opencv create a border around each strip that cut slightly into the image itself and removed everything outside of that. After that I had it overlay the strips and allowed for some manual adjustments (no deformations since that would cause major distortions due to the time difference between measuring each strip)
– Arno van der Weijden
11 hours ago






It's messy code but it works....we.tl/t-whtVSIWRFc My boss has the full version as an exe but I can't find the code for it anymore. I simply had opencv create a border around each strip that cut slightly into the image itself and removed everything outside of that. After that I had it overlay the strips and allowed for some manual adjustments (no deformations since that would cause major distortions due to the time difference between measuring each strip)
– Arno van der Weijden
11 hours ago














There are some minor distortions in each strip but they are very minor according to my measurements.
– Arno van der Weijden
11 hours ago




There are some minor distortions in each strip but they are very minor according to my measurements.
– Arno van der Weijden
11 hours ago










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